全部产品分类
品牌： |
Swant | 货号： |
AgCB100abs |
目录价： |
￥8138.00 | 规格： |
100 µg |
产品分类： |
Recombinant Protein | 研究领域： |
Calbindin |
产品概述： |
This recombinant protein has been purified by HPLC and migrates as a single band of approximately 28 kDa. The figure shows a gel stained with Coomassie-blue. For adsorption experiments aimed at proofing the specificity of the immunostaining, we suggest the following procedure: - Dilute 1 µl of one of the Swants antibodies against calbindin D-28k in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5'000). - Add 1 µg of the recombinant protein to 1 ml of the diluted antibody solution and mix well. - Incubate for at least 6 hours in the cold. - Apply to tissue-sections and incubate for 3 days. - Complete the immunohistochemical reaction as usual (biotinylated secondary antibody, Streptavidin-Cy2 (or other fluorescent dyes), or peroxidase (followed by DAB-H2O2). As a result, the immunostaining should be strongly reduced or even completely prevented. | 来源： |
Recombinant mouse |
靶点/蛋白质： |
HPLC-purified calbindin D-28k (recombinant, 100 g) produced in Escherichia Coli, after in vitro cDNA synthesis. | 特异性： |
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存储温度： |
After reconstitution, freeze in small aliquots (e.g. 1 to 10 ug) and keep at -80°C. Avoid repeated freezing and thawing. | 存储溶液： |
100 ug in 250 ul of 20 mM NaHCO3 with high CaCl2 (0.1 mM). Lyophilized. |
应用： |
This protein can be used for immuoblots and absorption experiments. For intracellular injections in neurons we suggest using our other products: AgCB10ic or AgCB100ic. | 参考文献： |
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详情介绍： |
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别名： |
说明书： |
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产品官网链接： |
https://www.swant.com/pdfs/AgCB100abs.pdf | 订购数量： |
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