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当前位置:首页 > 抗原抗体、ELISA、WB > 一抗 > Antibodies against Ca2+ -bindin > Monoclonal anti parvalbumin
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Monoclonal anti parvalbumin

品牌:

Swant

货号:

235-200 µl

目录价:

¥4787.00

规格:

200 µl

产品分类:

Antibodies against Ca2+ -bindin

研究领域:

Parvalbumin

产品概述:

Monoclonal anti-Parvalbumin is a mouse IgG1 produced by hybridization of mouse myeloma cells with spleen cells from mice immunized with parvalbumin purified from carp muscles (1). The antibody was evaluated for specificity and potency: a) by indirect immunofluorescent or immunoperoxidase labeling as well as Avidin-Biotin staining of cryostat or vibratome-sections of 4% paraformaldehyde fixed tissue; b) by immunoenzymatic labelling of immunoblots; c) by radioimmunoassay (RIA)(1). The product is a monoclonal antibody (McAB) against parvalbumin, a calcium-binding protein of the EF-hand family related to calmodulin and troponin-C. The antibody reacts specifically with parvalbumin in tissue originating from human, monkey, rabbit, rat, mouse chicken and fish. The McAB specifically stains the 45Ca-binding spot of parvalbumin (MW 12'000 and IEF 4.9) in a two-dimensional "immunoblot". In a RIA set up the McAB measures parvalbumin with a sensitivity of 10 ng/assay and an affinity of 7.9 x 1012 L/M (1).

来源:

Mouse monoclonal, IgG1

靶点/蛋白质:

Parvalbumin

特异性:

存储温度:

For long storage, keep small aliquots of 1-2 µl at - 80°C (or at least - 20°C). For continous use keep diluted solutions at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.

存储溶液:

Lyophilized ascites (no preservatives).

应用:

Immunohistochemistry: 1:5'000 - 1:10'000 Immunoblotting: 1:1'000. We recommend that the optimal dilution be determined by titration test.

参考文献:

1. Celio M.R. et al., Cell Calcium 9:81-86, 1988 2. Celio M.R., Heizmann C.W. (1981) Nature 293: 300-302 3. Celio M.R., Heizmann C.W. (1982) Nature 297:504-506 4. Celio M.R. (1986) Science 231:995-997 5. Schwaller B., et al. (1999) Am. J. Physiol. 276. C395-403

详情介绍:

别名:

说明书:

产品官网链接:

https://www.swant.com/pdfs/Monoclonal_parvalbumin_235.pdf

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