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Mouse anti-calretinin (monoclonal)

6B3-1 ml
货号 货期 规格 / 价格 询价

Mouse anti-calretinin (monoclonal)

品牌

Swant

货号

6B3-1 ml

产品分类

Antibodies against Ca2+ -bindin

研究领域

Calretinin

产品概述

After reconstitution freeze in small aliquots (e.g. 1 µl) and keep at - 80°C (or at least - 20°C).For continuous use keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.

形式

The antibody against calretinin was produced in mice by immunization with recombinant human calretinin-22k (1). Calretinin 22k is an alternative splice product of the calretinin gene and identical with calretinin up to Arg178. After fusion, hybridoma cells were screened with human recombinant calretinin as target and the clone 6B3 was selected. The antibody 6B3 recognizes an epitope within the first 4 EF-hands domains common to both calretinin and calretinin-22k (1). It was evaluated for specificity and potency: a) by Biotin-Avidin labeling of cryostate-, vibratome- and paraffin-sections of 4% paraformaldehyde (or 10% buffered formalin) fixed brains b) by immunoenzymatic labelling of immunoblots c) by immunohistochemistry on tissue of calretinin knock-out mice. The product is a monoclonal antibody against calretinin a calcium-binding protein of the EFhand family related to calbindin D-28k and calmodulin (2). The antibody reacts specifically with calretinin in tissue originating from human and rat. This antibody does not cross-react with calbindin D-28k or other known calcium binding-proteins, as determined by its distribution in the brain, as well as by immunoblots. The antibody recognizes calretinin in human mesotheliomas (3,4).

浓度

Calretinin

纯度

Mouse monoclonal

试剂准备

Lyophilized concentrated supernatant (no preservatives).

复溶

1. Zimmermann, L. and Schwaller B (2002) Monoclonal antibodies recognizes epitopes of calretinin. Cell Calcium 31: 13-25. 2. Rogers J.H. (1987) Calretinin: a gene for a novel calcium-binding protein expressed principally in neurons. J. Cell Biol. 105:1343-1353. 3. Gotzos V., Vogt P. and M.R. Celio (1995) Calretinin is a selective marker for malignant pleural mesotheliomas of the epithelial type. Pathol. Res. Pract. 192:137-147. 4. Doglioni, C. et al. (1996) Calretinin: a novel immunocytochemical marker for mesothelioma. Am. J. Surg. Pathol. 20:1037-1046. 5. Schiffmann S.N. et al (1999) Impaired motor coordination and Purkinje cell excitability in mice lacking calretinin. PNAS, 96: 5257-5262.

应用

Immunohistochemistry: 1:2'000 - 1:5'000. On paraformaldehyde (4%) or formalin-fixed tissue. Cryostate or paraffin-sections. Immunoblots: 1:1'000 - 1:2'000. For immunohistochemistry and immunoblots the titer was determined by using the avidinbiotin method. We recommend that the optimal dilutions be determined by titration experiments.

产品描述

Lot No: 010399 Reconstitution: each vial with 200 µl of bidistilled water. Immunoblot of brain extracts: Antibody 6B3 specifically stains the calretinin band on immunoblots of brain extracts (Fig. 1).Fig. 1 Immunoblot of brain homogenate of various species with the monoclonal antibody 6B3.1: Mouse; 2: Rat; 3. Guinea pig; 4. Rabbit; 5. Macaca fascicularis; 6. Zebrafish; 7. Chicken. In all species, a band at 29 KDa is detected. Immunohistochemistry on Calbindin D-28k knock-out mice: Antibody 6B3 immunolabels a subpopulation of neurons in the normal brain with highefficiency (Fig. 2a), but does not stain the brain of calretinin knock out mice (Fig. 2b). Product: Conc. supernat.

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