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Rabbit anti-Ca2+-ATPase, isoform 2

货号 货期 规格 / 价格 询价

Rabbit anti-Ca2+-ATPase, isoform 2

品牌

Swant

货号

PMCA2

产品分类

Antibodies against Ca2+ exchang

研究领域

Ca2+ - pumps

产品概述

After reconstitution freeze in small aliquots (e.g. 1 ul) and keep at - 80°C (or at least - 20°C). For continuous use keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.

形式

The antiserum against Ca2+-ATPase, isoform 2, was produced in rabbits by immunization with the N-terminal sequence-specific peptide of the human pump as epitope. It recognizes the corresponding proteins in human, rat and mouse tissue. The antibody was evaluated for specificity and potency : a) by Biotin-Avidin labelling of cryostatesections of 4% paraformaldehyde fixed tissue and b) by immunoenzymatic labelling of Western blots of brain membranes or primary nerve cell cultures. It can also be used for immunoprecipitation. The serum was inactivated at 50°C for 30 minutes.

浓度

Ca2+-ATPase 2 (human plasma membrane)

纯度

Rabbit

试剂准备

Lyophilized whole serum (no preservatives).

复溶

1. Stauffer T.P., Guerini D., Carafoli E. (1995) Tissue distribution of the four gene products of the plasma-membrane Ca2+-pump. A study using specific antibodies. J. Biol. Chem. 19: 12184- 12190. 2. Stauffer T.P., Guerini D., Celio M.R., Carafoli E. (1997) Immunolocalization of the plasma membrane Ca2+-pump isoforms in the rat brain. Brain Res. 748:21-29.

应用

Immunohistochemistry : 1: 1'000 - 1: 2'000. Immunoblots : 1: 1'000 - 1: 2'000. For immunohistochemistry and immunoblots the titer was determined by using the avidin-biotin method. We recommend that the optimal dilutions be determined by titration experiments.

产品描述

Product: Antiserum Ref: 10 Code No: PMCA2 Lot No: #73 Reconstitution: with 200 ul of bidistilled water

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