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Monoclonal anti-Na+ -Ca2+-exchanger

货号 货期 规格 / 价格 询价

Monoclonal anti-Na+ -Ca2+-exchanger

品牌

Swant

货号

R3F1

产品分类

Antibodies against Ca2+ exchang

研究领域

Ca2+ - exchangers

产品概述

After reconstitution freeze in small aliquots (e.g. 1 ul) and keep at - 80°C (or at least - 20°C). For continuous use keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.

形式

The monoclonal antibody against the canine Na+ -Ca2+-exchanger, recognizes two neighboring but non-overlapping sequences of the hydrophilic region of the exchanger, connecting the putative transmembrane segments 5 and 6. The antibody was evaluated for specificity and potency by immunoblotting, and immunoprecipitation (see reference). It recognizes virtually only NCX1 in monkey, horse, dog, rat, bovine and mouse tissue. At higher concentrations it reacts faintly with NCX2, whereas NCX3 is not recognized. Both splice variants with exon A (heart, nervous system) and exon B (kidney, glia) are recognized.

浓度

Na+-Ca2+ exchanger protein (cardiac)

纯度

Mouse monoclonal, IgG1

试剂准备

Lyophilized, concentrated supernatant.

复溶

Porzig H., Li Z., Nicoll D.A., Philipson K.D. (1993) Mapping the cardiac sodium-calcium exchanger with monoclonal antibodies. Am. J. Physiol. 265:C78 -C756, 1993

应用

Immunohistochemistry: 1: 500; 1: 1’000 Immunoblots and immunoprecipitation: 1: 500; 1: 1'000 (use milk for blocking). A strong 70 kDa band is found in addition to the 116 kDa of the integral exchanger. Often the HMW band is a double band 116/ 112 kDa band. The titer was determined using the indirect-peroxidase method. We recommend that the optimal dilutions be determined by titration experiments.

产品描述

Product: Conc. Supernat Ref: 9 Code No: R3F1 Lot No: #mr05 Reconstitution: with 200 ul of bidistilled water. IgG Subtype: IgG1

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